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STK39 Recombinant antibody

STK39 Recombinant Antibody for FC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230382G12

Cat No : 83081-3-RR

Print datasheet

Synonyms

DCHT, DKFZp686K05124, PASK, SPAK, Ste 20 related kinase, STK39

验证数据展示

  • IF Staining of HepG2 using 83081-3-RR

    IF Staining of HepG2 using 83081-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STK39 antibody (83081-3-RR, Clone: 230382G12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of Daudi using 83081-3-RR

    FC experiment of Daudi using 83081-3-RR

    1x10^6 Daudi cells were intracellularly stained with 0.25 ug Anti-Human STK39 (83081-3-RR, Clone:230382G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inDaudi cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83081-3-RR targets STK39 in IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen STK39 fusion protein Ag34503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Synonyms DCHT, DKFZp686K05124, PASK, SPAK, Ste 20 related kinase, STK39
Calculated Molecular Weight59 kDa
GenBank Accession NumberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UEW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

实验方案

Product Specific Protocols
IF protocol for STK39 antibody 83081-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols