验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.00 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82925-2-RR targets STMN2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | STMN2 fusion protein Ag0929 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | stathmin-like 2 |
Synonyms | SGC 10, SCGN10, SCGN 10, SCG10, SCG 10 |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 18-21 kDa |
GenBank Accession Number | BC006302 |
Gene Symbol | STMN2 |
Gene ID (NCBI) | 11075 |
RRID | AB_3670662 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q93045 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)
实验方案
Product Specific Protocols | |
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WB protocol for STMN2 antibody 82925-2-RR | Download protocol |
IHC protocol for STMN2 antibody 82925-2-RR | Download protocol |
IF protocol for STMN2 antibody 82925-2-RR | Download protocol |
IP protocol for STMN2 antibody 82925-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |