• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

STMN4 Polyclonal antibody, PBS Only

STMN4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12027-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human gliomas using 12027-1-AP (same clone as 12027-1-PBS)

    IHC staining of human gliomas using 12027-1-AP (same clone as 12027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

  • WB analysis of human brain using 12027-1-AP (same clone as 12027-1-PBS)

    WB analysis of human brain using 12027-1-AP (same clone as 12027-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12027-1-AP (STMN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12027-1-AP (same clone as 12027-1-PBS)

    IP experiment of mouse brain using 12027-1-AP (same clone as 12027-1-PBS)

    IP result of anti-STMN4 (IP:12027-1-AP, 3ug; Detection:12027-1-AP 1:1000) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

产品信息

12027-1-PBS targets STMN4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STMN4 fusion protein Ag2657 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stathmin-like 4
Synonyms
Calculated Molecular Weight 216 aa, 25 kDa
Observed Molecular Weight 22-30 kDa
GenBank Accession NumberBC011520
Gene Symbol STMN4
Gene ID (NCBI) 81551
RRIDAB_2197401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H169
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RB3 (Stathmin-4), encoded by STMN4 gene, is a neuron-specific homologue of the SCG10/stathmin family proteins, possessing a unique N-terminal membrane-associated domain and the stathmin-like domain at the C terminus, which promotes microtubule (MT) catastrophe and/or tubulin sequestering. Stathmin is a ubiquitous cytosolic phosphoprotein, preferentially expressed in the nervous system, and described as a relay integrating diverse intracellular signaling pathways. RB3 is consistently up-regulated in differentiated neuroblastoma cells, and stable expression of transfected RB3 could induce differentiation, suggesting a role of RB3 during neuroblastoma differentiation. Moreover, increased RB3 is a unique response protein in the stathmin-related proteins following optical nerve axotomy and RB3 may be a critical target for neurodegenerative diseases. STMN4 has 4 isoforms with MW 25, 23, 22 and 19 kDa (refer to UniProt). Another 30 kDa isoform can be found by NCBI Protein Blast. (https://www.ncbi.nlm.nih.gov/protein/XP_005273709)