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验证数据展示

  • WB analysis of HeLa using 12046-1-AP

    WB analysis of HeLa using 12046-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12046-1-AP

    IHC staining of human lung cancer using 12046-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STOM antibody included
  • IHC staining of human lung cancer using 12046-1-AP

    IHC staining of human lung cancer using 12046-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STOM antibody included
  • IF Staining of A549 using 12046-1-AP

    IF Staining of A549 using 12046-1-AP

    Immunofluorescent analysis of A549 cells using 12046-1-AP (STOM antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 12046-1-AP

    IP experiment of HeLa using 12046-1-AP

    IP result of anti-Stomatin (IP:12046-1-AP, 3ug; Detection:12046-1-AP 1:300) with HeLa cells lysate 2800ug.

Stomatin Polyclonal antibody

Stomatin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12046-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

STOM, Erythrocyte membrane protein band 7.2, EPB72, EPB7, BND7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy STOM Ready-To-Use IHC Kit. STOM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12046-1-AP targets Stomatin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human
文献引用反应性human
免疫原 Stomatin fusion protein Ag2676 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stomatin
别名 STOM, Erythrocyte membrane protein band 7.2, EPB72, EPB7, BND7
计算分子量 288 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC010703
基因名称 Stomatin
Gene ID (NCBI) 2040
RRIDAB_2197406
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

实验方案

Product Specific Protocols
WB protocol for Stomatin antibody 12046-1-APDownload protocol
IHC protocol for Stomatin antibody 12046-1-APDownload protocol
IF protocol for Stomatin antibody 12046-1-APDownload protocol
IP protocol for Stomatin antibody 12046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Med

Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome

Authors - Yashuang Yang
View Article
humanWB

Vox Sang

Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools.

Authors - Rubin O O
View Article
humanIHC

Chin J Cancer Res

Stomatin plays a suppressor role in non-small cell lung cancer metastasis.

Authors - Huaying An
View Article
humanWB,CoIP

Biophys J

Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains

Authors - Artem G Ayuyan
View Article