验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy STOM Ready-To-Use IHC Kit. STOM primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12046-1-AP targets Stomatin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Stomatin fusion protein Ag2676 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | stomatin |
| Synonyms | STOM, Erythrocyte membrane protein band 7.2, EPB72, EPB7, BND7 |
| Calculated Molecular Weight | 288 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC010703 |
| Gene Symbol | Stomatin |
| Gene ID (NCBI) | 2040 |
| RRID | AB_2197406 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27105 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Stomatin antibody 12046-1-AP | Download protocol |
| IHC protocol for Stomatin antibody 12046-1-AP | Download protocol |
| IF protocol for Stomatin antibody 12046-1-AP | Download protocol |
| IP protocol for Stomatin antibody 12046-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Med Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome | ||
Vox Sang Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools. | ||
Chin J Cancer Res Stomatin plays a suppressor role in non-small cell lung cancer metastasis. | ||
Biophys J Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains |
