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验证数据展示

  • WB analysis of Raji using 10688-1-AP

    WB analysis of Raji using 10688-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human heart using 10688-1-AP

    IHC staining of human heart using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 10688-1-AP

    IHC staining of human heart using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 40x lens).

STRADB Polyclonal antibody

STRADB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10688-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ALS2CR2, CALS 21, ILP interacting protein, ILPIP, ILPIPA, PAPK, PRO1038, Pseudokinase ALS2CR2, STRAD beta, STRADB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10688-1-AP targets STRADB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 STRADB fusion protein Ag1040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 STE20-related kinase adaptor beta
别名 ALS2CR2, CALS 21, ILP interacting protein, ILPIP, ILPIPA, PAPK, PRO1038, Pseudokinase ALS2CR2, STRAD beta, STRADB
计算分子量 47 kDa
观测分子量 28-31 kDa, 38-42 kDa
GenBank蛋白编号BC008302
基因名称 STRADB
Gene ID (NCBI) 55437
RRIDAB_2197715
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0K7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 418 amino acid STRADB protein, which is also named as ALS2CR2 and ILPIP, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. STRADB potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. This protein has 3 isoforms produced by alternative splicing with the MW of 47 kDa, 42 kDa and 31 kDa.

实验方案

Product Specific Protocols
WB protocol for STRADB antibody 10688-1-APDownload protocol
IHC protocol for STRADB antibody 10688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols