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STRADB Polyclonal antibody

STRADB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10688-1-AP

Print datasheet

Synonyms

ALS2CR2, CALS 21, ILP interacting protein, ILPIP, ILPIPA, PAPK, PRO1038, Pseudokinase ALS2CR2, STRAD beta, STRADB

验证数据展示

  • WB analysis of Raji using 10688-1-AP

    WB analysis of Raji using 10688-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human heart using 10688-1-AP

    IHC staining of human heart using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 10688-1-AP

    IHC staining of human heart using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inRaji cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10688-1-AP targets STRADB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STRADB fusion protein Ag1040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name STE20-related kinase adaptor beta
Synonyms ALS2CR2, CALS 21, ILP interacting protein, ILPIP, ILPIPA, PAPK, PRO1038, Pseudokinase ALS2CR2, STRAD beta, STRADB
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 28-31 kDa, 38-42 kDa
GenBank Accession NumberBC008302
Gene Symbol STRADB
Gene ID (NCBI) 55437
RRIDAB_2197715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0K7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 418 amino acid STRADB protein, which is also named as ALS2CR2 and ILPIP, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. STRADB potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. This protein has 3 isoforms produced by alternative splicing with the MW of 47 kDa, 42 kDa and 31 kDa.

实验方案

Product Specific Protocols
WB protocol for STRADB antibody 10688-1-APDownload protocol
IHC protocol for STRADB antibody 10688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols