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STRAP Monoclonal antibody
STRAP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5E11F3
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy STRAP Ready-To-Use IHC Kit. STRAP primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66712-1-Ig targets STRAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | STRAP fusion protein Ag13085 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | serine/threonine kinase receptor associated protein |
| Synonyms | MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC000162 |
| Gene Symbol | STRAP |
| Gene ID (NCBI) | 11171 |
| RRID | AB_2882063 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y3F4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STRAP antibody 66712-1-Ig | Download protocol |
| IHC protocol for STRAP antibody 66712-1-Ig | Download protocol |
| IF protocol for STRAP antibody 66712-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program |