验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, human placenta tissue |
| Positive IHC detected in | human placenta tissue, human hysteromyoma tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17870-1-AP targets STS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | STS fusion protein Ag12353 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | steroid sulfatase (microsomal), isozyme S |
| 别名 | ARSC, ARSC1, Arylsulfatase C, ASC, ES, SSDD, Steroid sulfatase, Steryl sulfatase, Steryl sulfate sulfohydrolase, STS |
| 计算分子量 | 583 aa, 65 kDa |
| 观测分子量 | 63-65 kDa |
| GenBank蛋白编号 | BC075030 |
| 基因名称 | STS |
| Gene ID (NCBI) | 412 |
| RRID | AB_2240080 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08842 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STS(Steroid sulfatase) is also named as ASC(arylsulfatase C), ARSC1 and belongs to the sulfatase family. It is expressed in human liver and is responsible for the hydrolysis of many estrogen and hydroxysteroid sulfates, including dehydroepiandrosterone (DHEA)-sulfate and β-estradiol(E2)-3-sulfate(PMID:19589875). This enzyme has also been documented to occur in many other tissues, including skin, lung, ovary, and adrenal gland. The estimated molecular mass of expressed human STS is 63-85 kDa which may represent different levels of glycosylation in the different tissues, as STS is known to be a glycoprotein and the full length protein has a signal peptide with 21 amino acids(PMID:17604157).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STS antibody 17870-1-AP | Download protocol |
| IHC protocol for STS antibody 17870-1-AP | Download protocol |
| IF protocol for STS antibody 17870-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Steroid Sulfatase Stimulates Intracrine Androgen Synthesis and is a Therapeutic Target for Advanced Prostate Cancer.
| ||
Sci Rep Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application. | ||
Mol Cell Biochem Resveratrol and its methoxy derivatives modulate the expression of estrogen metabolism enzymes in breast epithelial cells by AhR down-regulation. | ||
J Biomed Res Suppressed estrogen supply via extra-ovarian progesterone receptor membrane component 1 in menopause. | ||
Life Sci Alliance Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration |
