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STT3A Monoclonal antibody, PBS Only

STT3A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2B12

Cat no : 66581-1-PBS

Print datasheet

Synonyms

B5, Integral membrane protein 1, ITM1, STT3 A, STT3A, TMC, Transmembrane protein TMC

验证数据展示

  • WB analysis of THP-1 using 66581-1-Ig (same clone as 66581-1-PBS)

    WB analysis of THP-1 using 66581-1-Ig (same clone as 66581-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66581-1-Ig (STT3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

    IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66581-1-Ig (STT3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

产品信息

66581-1-PBS targets STT3A in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen STT3A fusion protein Ag27072 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms B5, Integral membrane protein 1, ITM1, STT3 A, STT3A, TMC, Transmembrane protein TMC
Calculated Molecular Weight 705 aa, 81 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC020965
Gene Symbol STT3A
Gene ID (NCBI) 3703
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46977
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STT3A, also named as Integral membrane protein 1, belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. There are two isoforms of STT3A with molecular weight of 81 and 70 kDa. 66581-1-Ig antibody detects a protein around 65-70 kDa in SDS-PAGE which is similar to papers published. (PMID: 32005703, 25135935)