STT3A Recombinant antibody
STT3A Recombinant Antibody for ELISA, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
ELISA, FC (Intra)
Conjugate
Unconjugated
CloneNo.
230426B10
验证数据展示
经过测试的应用
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83077-4-RR is STT3A Fusion Protein expressed in E. coli.
Tested Applications | ELISA, FC (Intra) Application Description |
Tested Reactivity | Human |
Immunogen | STT3A fusion protein Ag31994 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) |
Synonyms | B5, FLJ27038, Integral membrane protein 1, ITM1, STT3 A, STT3A, TMC, Transmembrane protein TMC |
Calculated Molecular Weight | 705 aa, 81 kDa |
GenBank Accession Number | BC020965 |
Gene Symbol | STT3A |
Gene ID (NCBI) | 3703 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P46977 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STT3A, also named as Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, is a 705 amino acid prtein, which belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.
实验方案
Product Specific Protocols | |
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FC protocol for STT3A antibody 83077-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |