验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, PC-12 cells, mouse kidney tissue, rat kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy STUB1 Ready-To-Use IHC Kit. STUB1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29751-1-AP targets STUB1 in WB, ELISA applications and shows reactivity with Human, Mouse , Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse , Rat |
| 免疫原 | STUB1 fusion protein Ag31335 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | STIP1 homology and U-box containing protein 1 |
| 别名 | Antigen NY CO 7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1 |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC007545 |
| 基因名称 | STUB1 |
| Gene ID (NCBI) | 10273 |
| RRID | AB_2923603 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNE7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STIP1 Homology and U-Box Containing Protein 1 (STUB1), formerly known as CHIP (C-terminus of HSP70-Interacting Protein), is a chaperone-dependent E3 ubiquitin ligase. STUB1 is a 34.5 kDa co-chaperone encoded by the STUB1 gene. STUB1 is highly conserved across species and is expressed in most tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STUB1 antibody 29751-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |