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Syntaxin 10 Polyclonal antibody

Syntaxin 10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11036-1-AP

Print datasheet

Synonyms

Syntaxin-10, Syn10, STX10

验证数据展示

  • IHC staining of mouse heart using 11036-1-AP

    IHC staining of mouse heart using 11036-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STX10 antibody included
  • IHC staining of mouse heart using 11036-1-AP

    IHC staining of mouse heart using 11036-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STX10 antibody included
  • WB analysis of HeLa using 11036-1-AP

    WB analysis of HeLa using 11036-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11036-1-AP (Syntaxin 10 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, BxPC-3 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STX10 Ready-To-Use IHC Kit. STX10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11036-1-AP targets Syntaxin 10 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Syntaxin 10 fusion protein Ag1501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin 10
Synonyms Syntaxin-10, Syn10, STX10
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017237
Gene Symbol Syntaxin 10
Gene ID (NCBI) 8677
RRIDAB_2198210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60499
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.

实验方案

Product Specific Protocols
WB protocol for Syntaxin 10 antibody 11036-1-APDownload protocol
IHC protocol for Syntaxin 10 antibody 11036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Eur J Immunol

SNARE protein expression and localization in human cytotoxic T lymphocytes.

Authors - Pattu Varsha V
View Article
humanWB

Front Oncol

Identification of a novel MYC target gene set signature for predicting the prognosis of osteosarcoma patients

Authors - Deliang Gong
View Article