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  • KD/KO Validated

Syntaxin 11 Polyclonal antibody

Syntaxin 11 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13301-1-AP

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Synonyms

Syntaxin-11, STX11



经过测试的应用

Positive WB detected inmouse lung tissue, DU 145 cells, human placenta tissue, human skeletal muscle tissue, Jurkat cells, L02 cells, mouse placenta tissue, rat lung tissue
Positive IP detected inDU 145 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STX11 Ready-To-Use IHC Kit. STX11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13301-1-AP targets Syntaxin 11 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Syntaxin 11 fusion protein Ag4097 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin 11
Synonyms Syntaxin-11, STX11
Calculated Molecular Weight 287 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC033519
Gene Symbol Syntaxin 11
Gene ID (NCBI) 8676
RRIDAB_2198214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75558
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin 11 (STX11) belongs to the syntaxin family. It interacts with the SNARE proteins SNAP-23 and VAMP. STX11 behaves as an integral membrane protein despite the lack of a traditional transmembrane domain. The detection of STX11 in human cytotoxic lymphocytes suggests that this protein could play a role in the process leading to degranulation of secretory lysosomes (PMID:17525286).

实验方案

Product Specific Protocols
WB protocol for Syntaxin 11 antibody 13301-1-APDownload protocol
IHC protocol for Syntaxin 11 antibody 13301-1-APDownload protocol
IP protocol for Syntaxin 11 antibody 13301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Extracell Vesicles

Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion

Authors - Chuqi Liu
  • KO Validated
humanWB

J Exp Med

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

Authors - Frank Cichocki
ratWB,IF

Proc Natl Acad Sci U S A

Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.

Authors - Bin Na-Ryum NR
humanWB,IF, IP

J Cell Biol

The septin cytoskeleton regulates natural killer cell lytic granule release.

Authors - Prasad V Phatarpekar
humanWB, IHC

J Cell Sci

Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain-1 of Munc18 is indispensable for mast cell exocytosis.

Authors - Na-Ryum Bin
humanWB

Front Immunol

Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.

Authors - Jamie A Lopez