验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
11201-1-AP targets Syntaxin 16 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Syntaxin 16 fusion protein Ag1657 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 16 |
| 别名 | Golgi Marker, hsyn16, STX16, SYN16, syntaxin 16 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC019042 |
| 基因名称 | Syntaxin 16 |
| Gene ID (NCBI) | 8675 |
| RRID | AB_2198362 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14662 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 16 antibody 11201-1-AP | Download protocol |
| IHC protocol for Syntaxin 16 antibody 11201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD. | ||
Eur J Immunol SNARE protein expression and localization in human cytotoxic T lymphocytes. | ||
Traffic Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport. | ||
Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. |