• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Y79 using 83298-1-RR

    WB analysis of Y79 using 83298-1-RR

    Y79 cells were subjected to SDS PAGE followed by western blot with 83298-1-RR (STX1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 83298-1-RR

    FC experiment of SH-SY5Y using 83298-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Stx1b Recombinant Antibody (83298-1-RR, Clone:240156B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IHC staining of mouse eye using 83298-1-RR

    IHC staining of mouse eye using 83298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IHC staining of mouse eye using 83298-1-RR

    IHC staining of mouse eye using 83298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IHC staining of mouse brain using 83298-1-RR

    IHC staining of mouse brain using 83298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IF Staining of mouse brain using 83298-1-RR

    IF Staining of mouse brain using 83298-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of mouse brain using 83298-1-RR

    IF Staining of mouse brain using 83298-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83298-1-RR

    Affinity and Kinetic Characterization of 83298-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83298-1-RR against Human STX1B were performed. The affinity constant is below 1 pM.

STX1B Recombinant antibody

STX1B Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83298-1-RR

产品说明书

CloneNo. 240156B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Syntaxin 1A, Syntaxin 1B, syntaxin 1B, STX1B2, STX1B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inmouse eye tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83298-1-RR targets STX1B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 STX1B fusion protein Ag10275 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 1B
别名 Syntaxin 1A, Syntaxin 1B, syntaxin 1B, STX1B2, STX1B1
计算分子量 288 aa, 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC062298
基因名称 STX1B
Gene ID (NCBI) 112755
RRIDAB_3670968
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61266
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).

实验方案

Product Specific Protocols
WB protocol for STX1B antibody 83298-1-RRDownload protocol
IHC protocol for STX1B antibody 83298-1-RRDownload protocol
IF protocol for STX1B antibody 83298-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols