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- KD/KO Validated
Syntaxin 4 Polyclonal antibody
Syntaxin 4 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, rat kidney tissue, HEK-293 cells, Jurkat cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 8 publications below |
产品信息
14988-1-AP targets Syntaxin 4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat |
Immunogen | Syntaxin 4 fusion protein Ag6973 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | syntaxin 4 |
Synonyms | Syntaxin-4, STX4A, STX4, Renal carcinoma antigen NY-REN-31 |
Calculated Molecular Weight | 34 kDa |
Observed Molecular Weight | 35-37 kDa |
GenBank Accession Number | BC002436 |
Gene Symbol | STX4 |
Gene ID (NCBI) | 6810 |
RRID | AB_2286910 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q12846 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
IHC protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
IP protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Brain Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction. | ||
Front Immunol Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing. | ||
Int J Biol Macromol PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode. | ||
Br J Pharmacol CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells. | ||
J Biol Chem Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin. | ||
J Transl Med Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure. |