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Syntaxin 6 Monoclonal antibody

Syntaxin 6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6G4

Cat no : 60059-1-Ig

Print datasheet

Synonyms

Syntaxin-6, STX6, 2B6G4

验证数据展示

  • WB analysis using 60059-1-Ig

    WB analysis using 60059-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 60059-1-Ig

    IHC staining of human brain using 60059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 60059-1-Ig

    IHC staining of human brain using 60059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inC6 cells, SH-SY5Y cells, U-251 cells, Jurkat cells, Neuro-2a cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60059-1-Ig targets Syntaxin 6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Syntaxin 6 fusion protein Ag1284 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name syntaxin 6
Synonyms Syntaxin-6, STX6, 2B6G4
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC009944
Gene Symbol Syntaxin 6
Gene ID (NCBI) 10228
RRIDAB_2196504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43752
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

实验方案

Product Specific Protocols
WB protocol for Syntaxin 6 antibody 60059-1-IgDownload protocol
IHC protocol for Syntaxin 6 antibody 60059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy

Authors - Shouheng Jin
View Article