验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, fetal human brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IP | See 1 publications below |
产品信息
66238-1-Ig targets STXBP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | STXBP2 fusion protein Ag7640 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin binding protein 2 |
| 别名 | Protein unc-18 homolog 2, pp10122, Munc18-2, MUNC18 2, Hunc18b |
| 计算分子量 | 593 aa, 66 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC002869 |
| 基因名称 | STXBP2 |
| Gene ID (NCBI) | 6813 |
| RRID | AB_2881627 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q15833 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STXBP2 antibody 66238-1-Ig | Download protocol |
| IHC protocol for STXBP2 antibody 66238-1-Ig | Download protocol |
| IF protocol for STXBP2 antibody 66238-1-Ig | Download protocol |
| IP protocol for STXBP2 antibody 66238-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity STING activation in platelets aggravates septic thrombosis by enhancing platelet activation and granule secretion |
