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验证数据展示

  • WB analysis of HepG2 using 13764-1-AP

    WB analysis of HepG2 using 13764-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 13764-1-AP

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lymphoma using 13764-1-AP

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of K-562 using 13764-1-AP

    IP experiment of K-562 using 13764-1-AP

    IP result of anti-STXBP3 (IP:13764-1-AP, 4ug; Detection:13764-1-AP 1:500) with K-562 cells lysate 1200ug.

STXBP3 Polyclonal antibody

STXBP3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13764-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C, PSP, STXBP3, syntaxin binding protein 3, UNC 18C, Unc18 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

13764-1-AP targets STXBP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 STXBP3 fusion protein Ag4747 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin binding protein 3
别名 MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C, PSP, STXBP3, syntaxin binding protein 3, UNC 18C, Unc18 3
计算分子量 592 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC038099
基因名称 STXBP3
Gene ID (NCBI) 6814
RRIDAB_2196832
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00186
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STXBP3 antibody 13764-1-APDownload protocol
IHC protocol for STXBP3 antibody 13764-1-APDownload protocol
IP protocol for STXBP3 antibody 13764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Diabetes

PMID: 20460426

Authors - Pontus Boström
View Article
mouseWB

J Biol Chem

microRNA-15b contributes to depression-like behavior in mice by affecting synaptic protein levels and function in the nucleus accumbens.

Authors - Li Guo
View Article
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
View Article