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验证数据展示

  • WB analysis using 24512-1-AP

    WB analysis using 24512-1-AP

    Mouse brain tissue was subjected to SDS PAGE, followed by western blot with 24512-1-AP (STXBP5 antibody) at a dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 24512-1-AP

    IP experiment of rat brain using 24512-1-AP

    IP result of anti-STXBP5 (IP:24512-1-AP, 5ug; Detection:24512-1-AP 1:300) with rat brain tissue lysate 4000ug.

  • IF Staining of SH-SY5Y using 24512-1-AP

    IF Staining of SH-SY5Y using 24512-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STXBP5 antibody (24512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STXBP5 Polyclonal antibody

STXBP5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24512-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Tomosyn-1, Syntaxin-binding protein 5, LLGL3, Lethal(2) giant larvae protein homolog 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inrat brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24512-1-AP targets STXBP5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 STXBP5 fusion protein Ag21629 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin binding protein 5 (tomosyn)
别名 Tomosyn-1, Syntaxin-binding protein 5, LLGL3, Lethal(2) giant larvae protein homolog 3
计算分子量 1151 aa, 128 kDa
观测分子量 130 kDa
GenBank蛋白编号BC113382
基因名称 STXBP5
Gene ID (NCBI) 134957
RRIDAB_2879582
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T5C0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-binding protein 5 (STXBP5, also known as tomosyn) is a cytosolic syntaxin-binding protein that can displace MUNC18 from syntaxin-1. It is a soluble R-SNARE protein that sequesters target SNAREs through the C-terminal VAMP-like domain. STXBP5 plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release.

实验方案

Product Specific Protocols
WB protocol for STXBP5 antibody 24512-1-APDownload protocol
IF protocol for STXBP5 antibody 24512-1-APDownload protocol
IP protocol for STXBP5 antibody 24512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

The syntaxin-binding protein STXBP5 regulates progerin expression

Authors - Hongqian Qi
  • KD Validated
View Article