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STXBP6 Polyclonal antibody

STXBP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10976-4-AP

Print datasheet

Synonyms

Amisyn, Syntaxin-binding protein 6, STXBP 6, HSPC156

验证数据展示

  • WB analysis of mouse brain using 10976-4-AP

    WB analysis of mouse brain using 10976-4-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 10976-4-AP

    WB analysis using 10976-4-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10976-4-AP

    IHC staining of human lung cancer using 10976-4-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 10976-4-AP

    IHC staining of human lung cancer using 10976-4-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human heart tissue, human brain tissue, BxPC-3 cells, rat brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10976-4-AP targets STXBP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen STXBP6 fusion protein Ag1427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin binding protein 6 (amisyn)
Synonyms Amisyn, Syntaxin-binding protein 6, STXBP 6, HSPC156
Calculated Molecular Weight 25 kDa or 45 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC009499
Gene Symbol STXBP6
Gene ID (NCBI) 29091
RRIDAB_2197002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STXBP6 antibody 10976-4-APDownload protocol
IHC protocol for STXBP6 antibody 10976-4-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB

BMC Med

A functional variant rs912304 for late-onset T1D risk contributes to islet dysfunction by regulating proinflammatory cytokine-responsive gene STXBP6 expression

Authors - Yu Qian
View Article