• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

STYK1 Polyclonal antibody

STYK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18028-1-AP

Print datasheet

Synonyms

NOK, Protein PK unique, STYK1, SuRTK106, Tyrosine protein kinase STYK1

验证数据展示

  • WB analysis of human brain using 18028-1-AP

    WB analysis of human brain using 18028-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18028-1-AP (STYK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 18028-1-AP

    IHC staining of mouse brain using 18028-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18028-1-AP (STYK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 18028-1-AP

    IHC staining of mouse brain using 18028-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18028-1-AP (STYK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

18028-1-AP targets STYK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen STYK1 fusion protein Ag12594 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine/tyrosine kinase 1
Synonyms NOK, Protein PK unique, STYK1, SuRTK106, Tyrosine protein kinase STYK1
Calculated Molecular Weight 422 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC093822
Gene Symbol STYK1
Gene ID (NCBI) 55359
RRIDAB_10603477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6J9G0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STYK1 antibody 18028-1-APDownload protocol
IHC protocol for STYK1 antibody 18028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oncol Lett

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Authors - Weiye Shi
View Article
humanWB

Heliyon

STYK1/NOK affects cell cycle late mitosis and directly interacts with anaphase-promoting complex activator CDH1

Authors - Su-Lin Zeng
View Article
mouseWB

Biochem Biophys Res Commun

Induction of chronic lymphocytic leukemia-like disease in STYK1/NOK transgenic mice

Authors - Yin Yang
View Article