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验证数据展示

  • WB analysis using 16433-1-AP

    WB analysis using 16433-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16433-1-AP (STYXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16433-1-AP

    IF Staining of HeLa using 16433-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STYXL1 antibody (16433-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

STYXL1 Polyclonal antibody

STYXL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16433-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

MKSTYX, MK STYX, Map kinase phosphatase-like protein MK-STYX, Inactive dual specificity protein phosphatase MK-STYX, DUSP24

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, PC-12 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16433-1-AP targets STYXL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 STYXL1 fusion protein Ag9743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine/threonine/tyrosine interacting-like 1
别名 MKSTYX, MK STYX, Map kinase phosphatase-like protein MK-STYX, Inactive dual specificity protein phosphatase MK-STYX, DUSP24
计算分子量 313 aa, 36 kDa
观测分子量25-36 kDa
GenBank蛋白编号BC024035
基因名称 STYXL1
Gene ID (NCBI) 51657
RRIDAB_3085490
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6J8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STYXL1, Serine/threonine/tyrosine-interacting-like protein 1, is an inner mitochondrial membrane protein that plays a role in induction of stimulus-dependent apoptosis via the intrinsic apoptotic pathway (PMID: 21262771). STYXL1 catalyzes inactive phosphatase (PMID: 20180778, PMID: 23163895). By binding to G3BP1, STYXL1 inhibits the formation of G3BP1-induced stress granules (PMID: 20180778, PMID: 23163895).

实验方案

Product Specific Protocols
WB protocol for STYXL1 antibody 16433-1-APDownload protocol
IF protocol for STYXL1 antibody 16433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Int J Biol Sci

LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.

Authors - Yan Fang
View Article
mouseWB

Int Immunopharmacol

Linderalactone mitigates diabetic cardiomyopathy in mice via suppressing the MAPK/ATF6 pathway

Authors - Xue Han
View Article