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验证数据展示

  • WB analysis using 27438-1-AP

    WB analysis using 27438-1-AP

    HT-1080 cell lysates were subjected to SDS PAGE followed by western blot with 27438-1-AP (SULF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 27438-1-AP

    IHC staining of mouse skeletal muscle using 27438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27438-1-AP (SULF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 27438-1-AP

    IHC staining of mouse skeletal muscle using 27438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27438-1-AP (SULF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SULF1 Polyclonal antibody

SULF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27438-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Extracellular sulfatase Sulf-2 secreted form, Extracellular sulfatase Sulf-1, Extracellular sulfatase Sulf 1, EC:3.1.6.14, EC:3.1.6.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27438-1-AP targets SULF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SULF1 fusion protein Ag26758 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfatase 1
别名 Extracellular sulfatase Sulf-2 secreted form, Extracellular sulfatase Sulf-1, Extracellular sulfatase Sulf 1, EC:3.1.6.14, EC:3.1.6.1
观测分子量101 kDa
GenBank蛋白编号BC068565
基因名称 SULF1
Gene ID (NCBI) 23213
RRIDAB_3085960
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWU6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SULF1 antibody 27438-1-APDownload protocol
IHC protocol for SULF1 antibody 27438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols