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验证数据展示

  • WB analysis using 19499-1-AP

    WB analysis using 19499-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 19499-1-AP

    IHC staining of human hepatocirrhosis using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 19499-1-AP

    IHC staining of human hepatocirrhosis using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of COLO 320 using 19499-1-AP

    IP experiment of COLO 320 using 19499-1-AP

    IP result of anti-SULT1A3 (IP:19499-1-AP, 4ug; Detection:19499-1-AP 1:200) with COLO 320 cells lysate 2240ug.

SULT1A3 Polyclonal antibody

SULT1A3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 19499-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

Sulfotransferase 1A3/1A4, SULT1 A3, SULT1A 3, SULT1A4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, Caco-2 cells, COLO 320 cells, HepG2 cells, HuH-7 cells, MCF-7 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

19499-1-AP targets SULT1A3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
别名 Sulfotransferase 1A3/1A4, SULT1 A3, SULT1A 3, SULT1A4
计算分子量 34 kDa
观测分子量 30-34 kDa
GenBank蛋白编号NM_177552
基因名称 SULT1A3
Gene ID (NCBI) 6818
RRIDAB_10642560
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0DMM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

实验方案

Product Specific Protocols
WB protocol for SULT1A3 antibody 19499-1-APDownload protocol
IHC protocol for SULT1A3 antibody 19499-1-APDownload protocol
IP protocol for SULT1A3 antibody 19499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Lett

Expression of sulfotransferases and sulfatases in human breast cancer: impact on resveratrol metabolism.

Authors - Miksits Michaela M
View Article