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验证数据展示

  • WB analysis using 16050-1-AP

    WB analysis using 16050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16050-1-AP

    IHC staining of human liver cancer using 16050-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human colon cancer using 16050-1-AP

    IHC staining of human colon cancer using 16050-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:240 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SULT1B1 Polyclonal antibody

SULT1B1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SULT1B2, ST1B2, ST1B1, EC:2.8.2.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, human colon tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:120-1:480
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16050-1-AP targets SULT1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SULT1B1 fusion protein Ag9009 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfotransferase family, cytosolic, 1B, member 1
别名 SULT1B2, ST1B2, ST1B1, EC:2.8.2.1
计算分子量 296 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC010895
基因名称 SULT1B1
Gene ID (NCBI) 27284
RRIDAB_2302767
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43704
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).

实验方案

Product Specific Protocols
WB protocol for SULT1B1 antibody 16050-1-APDownload protocol
IHC protocol for SULT1B1 antibody 16050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols