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SULT1B1 Polyclonal antibody

SULT1B1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16050-1-AP

Print datasheet

Synonyms

ST1B1, ST1B2, Sulfotransferase 1B1, Sulfotransferase 1B2, SULT1B1, SULT1B2

验证数据展示

  • WB analysis using 16050-1-AP

    WB analysis using 16050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16050-1-AP

    IHC staining of human liver cancer using 16050-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human colon cancer using 16050-1-AP

    IHC staining of human colon cancer using 16050-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 10x lens).

经过测试的应用

Positive WB detected inmouse colon tissue, human colon tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16050-1-AP targets SULT1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SULT1B1 fusion protein Ag9009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfotransferase family, cytosolic, 1B, member 1
Synonyms ST1B1, ST1B2, Sulfotransferase 1B1, Sulfotransferase 1B2, SULT1B1, SULT1B2
Calculated Molecular Weight 296 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC010895
Gene Symbol SULT1B1
Gene ID (NCBI) 27284
RRIDAB_2302767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43704
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).

实验方案

Product Specific Protocols
WB protocol for SULT1B1 antibody 16050-1-APDownload protocol
IHC protocol for SULT1B1 antibody 16050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols