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SULT1B1 Recombinant antibody

SULT1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240119C12

Cat no : 83228-1-RR

Print datasheet

Synonyms

SULT1B2, ST1B2, ST1B1, EC:2.8.2.1, 240119C12

验证数据展示

  • WB analysis of mouse colon using 83228-1-RR

    WB analysis of mouse colon using 83228-1-RR

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 83228-1-RR (SULT1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 83228-1-RR

    IHC staining of mouse colon using 83228-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 83228-1-RR

    IHC staining of mouse colon using 83228-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 83228-1-RR

    FC experiment of A431 using 83228-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SULT1B1 Recombinant antibody (83228-1-RR, Clone:240119C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83228-1-RR

    Affinity and Kinetic Characterization of 83228-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83228-1-RR against Human SULT1B1 were performed. The affinity constant is 63.8 nM.

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83228-1-RR targets SULT1B1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen SULT1B1 fusion protein Ag9009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sulfotransferase family, cytosolic, 1B, member 1
Synonyms SULT1B2, ST1B2, ST1B1, EC:2.8.2.1, 240119C12
Calculated Molecular Weight 296 aa, 35 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC010895
Gene Symbol SULT1B1
Gene ID (NCBI) 27284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43704
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).

实验方案

Product Specific Protocols
WB protocol for SULT1B1 antibody 83228-1-RRDownload protocol
IHC protocol for SULT1B1 antibody 83228-1-RRDownload protocol
FC protocol for SULT1B1 antibody 83228-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols