验证数据展示
经过测试的应用
| Positive WB detected in | human lung tissue, human adrenal gland tissue, human kidney tissue, human liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12522-1-AP targets SULT1E1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SULT1E1 fusion protein Ag3216 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sulfotransferase family 1E, estrogen-preferring, member 1 |
| 别名 | EST, EST 1, Estrogen sulfotransferase, ST1E1, STE, Sulfotransferase 1E1, SULT1E1 |
| 计算分子量 | 294 aa, 35 kDa |
| 观测分子量 | 32-35 kDa |
| GenBank蛋白编号 | BC027956 |
| 基因名称 | SULT1E1 |
| Gene ID (NCBI) | 6783 |
| RRID | AB_2302772 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49888 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SULT1E1 antibody 12522-1-AP | Download protocol |
| IHC protocol for SULT1E1 antibody 12522-1-AP | Download protocol |
| IP protocol for SULT1E1 antibody 12522-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol Inhibition of Estrogen Sulfotransferase (SULT1E1/EST) Ameliorates Ischemic Acute Kidney Injury in Mice. | ||
Biochem Pharmacol PXR phosphorylated at Ser350 transduces a glucose signal to repress the estrogen sulfotransferase gene in human liver cells and fasting signal in mouse livers. | ||
J Biol Chem Estrogen sulfotransferase is an oxidative stress responsive gene that gender-specifically affects liver ischemia/reperfusion injury.
| ||
Biochem Pharmacol Farnesoid X receptor regulates SULT1E1 expression through inhibition of PGC1α binding to HNF4α. | ||
