验证数据展示
经过测试的应用
| Positive WB detected in | mouse skin tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IHC detected in | human prostate cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12879-1-AP targets SULT2B1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SULT2B1 fusion protein Ag3838 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sulfotransferase family, cytosolic, 2B, member 1 |
| 别名 | Sulfotransferase family 2B member 1, ST2B1, HSST2, EC:2.8.2.2, ARCI14 |
| 计算分子量 | 365 aa, 41 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC034694 |
| 基因名称 | SULT2B1 |
| Gene ID (NCBI) | 6820 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00204 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. This antibody recognizes both of SULT2B1a and SULT2B1b.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SULT2B1 antibody 12879-1-AP | Download protocol |
| IHC protocol for SULT2B1 antibody 12879-1-AP | Download protocol |
| IP protocol for SULT2B1 antibody 12879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Lipid Res Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol. | ||
Drug Metab Dispos Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature. |
