SULT2B1 Polyclonal antibody
SULT2B1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse skin tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IHC detected in | human prostate cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12879-1-AP targets SULT2B1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SULT2B1 fusion protein Ag3838 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sulfotransferase family, cytosolic, 2B, member 1 |
| Synonyms | Sulfotransferase family 2B member 1, ST2B1, HSST2, EC:2.8.2.2, ARCI14 |
| Calculated Molecular Weight | 365 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC034694 |
| Gene Symbol | SULT2B1 |
| Gene ID (NCBI) | 6820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00204 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. This antibody recognizes both of SULT2B1a and SULT2B1b.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SULT2B1 antibody 12879-1-AP | Download protocol |
| IHC protocol for SULT2B1 antibody 12879-1-AP | Download protocol |
| IP protocol for SULT2B1 antibody 12879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Lipid Res Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol. | ||
Drug Metab Dispos Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature. |
