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SULT2B1 Polyclonal antibody

SULT2B1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29185-1-AP

Print datasheet

Synonyms

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1

验证数据展示

  • WB analysis using 29185-1-AP

    WB analysis using 29185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29185-1-AP (SULT2B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, mouse skin tissue, LNCaP cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29185-1-AP targets SULT2B1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen SULT2B1 fusion protein Ag30209 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfotransferase family, cytosolic, 2B, member 1
Synonyms Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1
Calculated Molecular Weight 365 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC034694
Gene Symbol SULT2B1
Gene ID (NCBI) 6820
RRIDAB_2918245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate.

实验方案

Product Specific Protocols
WB protocol for SULT2B1 antibody 29185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols