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验证数据展示

  • WB analysis using 29185-1-AP

    WB analysis using 29185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29185-1-AP (SULT2B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SULT2B1 Polyclonal antibody

SULT2B1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29185-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IF/ICC, ELISA

别名

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, mouse skin tissue, LNCaP cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29185-1-AP targets SULT2B1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 SULT2B1 fusion protein Ag30209 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfotransferase family, cytosolic, 2B, member 1
别名 Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1
计算分子量 365 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC034694
基因名称 SULT2B1
Gene ID (NCBI) 6820
RRIDAB_2918245
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00204
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate.

实验方案

Product Specific Protocols
WB protocol for SULT2B1 antibody 29185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols