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验证数据展示

  • WB analysis using 11210-1-AP

    WB analysis using 11210-1-AP

    A431 cell lysates were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11210-1-AP

    IHC staining of human breast cancer using 11210-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11210-1-AP (SUMF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11210-1-AP

    IHC staining of human breast cancer using 11210-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11210-1-AP (SUMF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A431 using 11210-1-AP

    IP experiment of A431 using 11210-1-AP

    IP result of anti-SUMF2 (IP:11210-1-AP, 4ug; Detection:11210-1-AP 1:2000) with A431 cells lysate 2640 ug.

  • IP experiment of HeLa using 11210-1-AP

    IP experiment of HeLa using 11210-1-AP

    IP result of anti-SUMF2 (IP:11210-1-AP, 4ug; Detection:11210-1-AP 1:2000) with HeLa cells lysate 1560 ug.

  • IF Staining of A431 using 11210-1-AP

    IF Staining of A431 using 11210-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SUMF2 antibody (11210-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SUMF2 Polyclonal antibody

SUMF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11210-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Sulfatase-modifying factor 2, sulfatase modifying factor 2, PSEC0171, pFGE, Paralog of formylglycine-generating enzyme

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, human skin tissue, human spleen tissue
Positive IP detected inA431 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11210-1-AP targets SUMF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 SUMF2 fusion protein Ag1711 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfatase modifying factor 2
别名 Sulfatase-modifying factor 2, sulfatase modifying factor 2, PSEC0171, pFGE, Paralog of formylglycine-generating enzyme
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC015600
基因名称 SUMF2
Gene ID (NCBI) 25870
RRIDAB_2255511
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SUMF2 antibody 11210-1-APDownload protocol
IHC protocol for SUMF2 antibody 11210-1-APDownload protocol
IF protocol for SUMF2 antibody 11210-1-APDownload protocol
IP protocol for SUMF2 antibody 11210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC

Int J Clin Exp Pathol

Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation.

Authors - Chuanfeng Fang
View Article
humanWB,IHC

Front Immunol

Advancing bladder cancer management: development of a prognostic model and personalized therapy

Authors - Xiang Huang
View Article