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验证数据展示

  • WB analysis using 10329-1-AP (same clone as 10329-1-PBS)

    WB analysis using 10329-1-AP (same clone as 10329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10329-1-AP (SUMO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 10329-1-AP (same clone as 10329-1-PBS)

    IHC staining of human testis using 10329-1-AP (same clone as 10329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SUMO1 antibody included
  • IHC staining of human testis using 10329-1-AP (same clone as 10329-1-PBS)

    IHC staining of human testis using 10329-1-AP (same clone as 10329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SUMO1 antibody included
  • IP experiment of HeLa using 10329-1-AP (same clone as 10329-1-PBS)

    IP experiment of HeLa using 10329-1-AP (same clone as 10329-1-PBS)

    IP result of anti-SUMO1 (IP:10329-1-AP, 4ug; Detection:10329-1-AP 1:800) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 10329-1-AP (same clone as 10329-1-PBS)

    IF Staining of A549 using 10329-1-AP (same clone as 10329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (10329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 10329-1-AP (same clone as 10329-1-PBS)

    FC experiment of A549 using 10329-1-AP (same clone as 10329-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human SUMO1 (10329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

SUMO1 Polyclonal antibody, PBS Only

SUMO1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 10329-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

Small ubiquitin-related modifier 1, Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SUMO1 Ready-To-Use IHC Kit. SUMO1 primary antibody included.

产品信息

10329-1-PBS targets SUMO1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SUMO1 fusion protein Ag0414 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
别名 Small ubiquitin-related modifier 1, Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1
计算分子量 12 kDa
观测分子量12~18 kDa, 80-90 kDa
GenBank蛋白编号BC006462
基因名称 SUMO1
Gene ID (NCBI) 7341
RRIDAB_2286872
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63165
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).