SUMO1 Monoclonal antibody

SUMO1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67559-1-Ig

产品说明书

CloneNo. 3B5B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, GAP-modifying protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse kidney tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy SUMO1 Ready-To-Use IHC Kit. SUMO1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67559-1-Ig targets SUMO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SUMO1 fusion protein Ag29402 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
别名 Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, GAP-modifying protein 1
计算分子量 12 kDa
观测分子量 15-100 kDa
GenBank蛋白编号BC006462
基因名称 SUMO1
Gene ID (NCBI) 7341
RRIDAB_2882773
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP63165
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.This antibody can detect endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 80-90 kD).

实验方案

Product Specific Protocols
WB protocol for SUMO1 antibody 67559-1-IgDownload protocol
IHC protocol for SUMO1 antibody 67559-1-IgDownload protocol
IF protocol for SUMO1 antibody 67559-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP

Biomed Pharmacother

Hydrogen sulfide regulates SERCA2a SUMOylation by S-Sulfhydration of SENP1 to ameliorate cardiac systole-diastole function in diabetic cardiomyopathy

Authors - Shuo Peng
humanWB

PLoS One

Isoginkgetin and Madrasin are poor splicing inhibitors

Authors - Michael Tellier
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