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验证数据展示

  • WB analysis of HEK-293 using 67154-1-Ig (same clone as 67154-1-PBS)

    WB analysis of HEK-293 using 67154-1-Ig (same clone as 67154-1-PBS)

    WB result of SUMO2/3 antibody (67154-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • WB analysis using 67154-1-Ig (same clone as 67154-1-PBS)

    WB analysis using 67154-1-Ig (same clone as 67154-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

    IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

    IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SUMO2/3 Monoclonal antibody, PBS Only

SUMO2/3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67154-1-PBS

产品说明书

CloneNo. 1A1B3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67154-1-PBS targets SUMO2/3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SUMO2/3 fusion protein Ag28672 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
别名 SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2
计算分子量 11 kDa
观测分子量 18 kDa
GenBank蛋白编号BC008450
基因名称 SUMO2
Gene ID (NCBI) 6613
RRIDAB_2882451
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61956
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.