验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
27556-1-AP targets SUN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | SUN2 fusion protein Ag16077 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | unc-84 homolog B (C. elegans) |
| 别名 | UNC84B, FRIGG, KIAA0668, Protein unc-84 homolog B, Rab5-interacting protein |
| 计算分子量 | 717 aa, 80 kDa |
| 观测分子量 | 80-85 kDa |
| GenBank蛋白编号 | BC111717 |
| 基因名称 | SUN2 |
| Gene ID (NCBI) | 25777 |
| RRID | AB_2880906 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UH99 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUN2 antibody 27556-1-AP | Download protocol |
| IHC protocol for SUN2 antibody 27556-1-AP | Download protocol |
| IF protocol for SUN2 antibody 27556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite | ||
Virology Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection |
