验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
27556-1-AP targets SUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | SUN2 fusion protein Ag16077 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | unc-84 homolog B (C. elegans) |
| Synonyms | UNC84B, SUN domain-containing protein 2, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B |
| Calculated Molecular Weight | 717 aa, 80 kDa |
| Observed Molecular Weight | 80-85 kDa |
| GenBank Accession Number | BC111717 |
| Gene Symbol | SUN2 |
| Gene ID (NCBI) | 25777 |
| RRID | AB_2880906 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UH99 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUN2 antibody 27556-1-AP | Download protocol |
| IF protocol for SUN2 antibody 27556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite | ||
Virology Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection |
