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验证数据展示

  • WB analysis using 68243-1-Ig (same clone as 68243-1-PBS)

    WB analysis using 68243-1-Ig (same clone as 68243-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68243-1-Ig (SUPT16H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 68243-1-Ig (same clone as 68243-1-PBS)

    WB analysis of MCF-7 using 68243-1-Ig (same clone as 68243-1-PBS)

    WB result of SUPT16H antibody (68243-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUPT16H transfected MCF-7 cells. This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 68243-1-Ig (same clone as 68243-1-PBS)

    IHC staining of human colon using 68243-1-Ig (same clone as 68243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68243-1-Ig (SUPT16H antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SUPT16H antibody included
  • IF Staining of HeLa using 68243-1-Ig (same clone as 68243-1-PBS)

    IF Staining of HeLa using 68243-1-Ig (same clone as 68243-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPT16H antibody (68243-1-Ig, Clone: 1E4C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SUPT16H Monoclonal antibody, PBS Only

SUPT16H Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68243-1-PBS

产品说明书

CloneNo. 1E4C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FACT140, FACT 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, CDC68

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SUPT16H Ready-To-Use IHC Kit. SUPT16H primary antibody included.

产品信息

68243-1-PBS targets SUPT16H in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SUPT16H fusion protein Ag29459 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 suppressor of Ty 16 homolog (S. cerevisiae)
别名 FACT140, FACT 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, CDC68
计算分子量 120 kDa
观测分子量135 kDa
GenBank蛋白编号NM_007192
基因名称 SUPT16H
Gene ID (NCBI) 11198
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y5B9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUPT16H, also named as FACT140, FACTP140, SPT16 and CDC68, belongs to the peptidase M24 family and SPT16 subfamily. SUPT16H is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). It also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.