- Featured Product
- KD/KO Validated
SUPT5H Polyclonal antibody
SUPT5H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
16511-1-AP targets SUPT5H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SUPT5H fusion protein Ag9728 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | suppressor of Ty 5 homolog (S. cerevisiae) |
| Synonyms | SPT5, hSPT5, DSIF p160, DSIF large subunit, DRB sensitivity-inducing factor large subunit |
| Calculated Molecular Weight | 1087 aa, 121 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC024203 |
| Gene Symbol | SUPT5H |
| Gene ID (NCBI) | 6829 |
| RRID | AB_2878268 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00267 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SUPT5H, also named as DSIF p160, is a 1087 amino acid protein, which belongs to the SPT5 family. SUPT5H localizes in the nucleus and is ubiquitously expressed. SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II.
SUPT5H is modified through phosphorylation and methylated, so the molecular weight of SUPT5H might be 140-150 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| IHC protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| IF protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| IP protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint | ||
Mol Cell SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.
| ||
Mol Cell INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II | ||
Mol Cell CDK7 kinase activity promotes RNA polymerase II promoter escape by facilitating initiation factor release | ||
