验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
16511-1-AP targets SUPT5H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SUPT5H fusion protein Ag9728 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | suppressor of Ty 5 homolog (S. cerevisiae) |
| 别名 | SPT5, hSPT5, DSIF p160, DSIF large subunit, DRB sensitivity-inducing factor large subunit |
| 计算分子量 | 1087 aa, 121 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC024203 |
| 基因名称 | SUPT5H |
| Gene ID (NCBI) | 6829 |
| RRID | AB_2878268 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00267 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SUPT5H, also named as DSIF p160, is a 1087 amino acid protein, which belongs to the SPT5 family. SUPT5H localizes in the nucleus and is ubiquitously expressed. SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H is modified through phosphorylation and methylated, so the molecular weight of SUPT5H might be 140-150 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| IHC protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| IF protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| IP protocol for SUPT5H antibody 16511-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint | ||
Mol Cell SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.
| ||
Mol Cell INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II | ||
Mol Cell CDK7 kinase activity promotes RNA polymerase II promoter escape by facilitating initiation factor release | ||
