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SUPT7L Polyclonal antibody

SUPT7L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25606-1-AP

Print datasheet

Synonyms

Adenocarcinoma antigen ART1, ART1, KIAA0764, SPT7L, STAF65, STAF65(gamma), STAF65gamma, STAGA complex 65 subunit gamma, Suppressor of Ty 7 like, SUPT7L

验证数据展示

  • IHC staining of human brain using 25606-1-AP

    IHC staining of human brain using 25606-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT7L antibody included
  • IHC staining of human brain using 25606-1-AP

    IHC staining of human brain using 25606-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT7L antibody included
  • WB analysis of HeLa using 25606-1-AP

    WB analysis of HeLa using 25606-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25606-1-AP (SUPT7L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25606-1-AP

    IF Staining of HeLa using 25606-1-AP

    Immunofluorescent analysis of HeLa cells using 25606-1-AP (SUPT7L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue, human gliomas tissue, rat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SUPT7L Ready-To-Use IHC Kit. SUPT7L primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25606-1-AP is SUPT7L Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SUPT7L fusion protein Ag22264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppressor of Ty 7 (S. cerevisiae)-like
Synonyms Adenocarcinoma antigen ART1, ART1, KIAA0764, SPT7L, STAF65, STAF65(gamma), STAF65gamma, STAGA complex 65 subunit gamma, Suppressor of Ty 7 like, SUPT7L
Calculated Molecular Weight 414 aa, 46 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC074000
Gene Symbol SUPT7L
Gene ID (NCBI) 9913
RRIDAB_2880156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUPT7L, also named as KIAA0764, STAF65gamma, is a component of the STAGA transcription coactivator-HAT complex. The MW of SUPT7L is 46-65 kDa.

实验方案

Product Specific Protocols
WB protocol for SUPT7L antibody 25606-1-APDownload protocol
IHC protocol for SUPT7L antibody 25606-1-APDownload protocol
IF protocol for SUPT7L antibody 25606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Genet

Loss-of-function variants affecting the STAGA complex component SUPT7L cause a developmental disorder with generalized lipodystrophy

Authors - Johannes Kopp
View Article