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验证数据展示

  • IHC staining of human brain using 25606-1-AP

    IHC staining of human brain using 25606-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT7L antibody included
  • IHC staining of human brain using 25606-1-AP

    IHC staining of human brain using 25606-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT7L antibody included
  • WB analysis of HeLa using 25606-1-AP

    WB analysis of HeLa using 25606-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25606-1-AP (SUPT7L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25606-1-AP

    IF Staining of HeLa using 25606-1-AP

    Immunofluorescent analysis of HeLa cells using 25606-1-AP (SUPT7L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SUPT7L Polyclonal antibody

SUPT7L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25606-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Adenocarcinoma antigen ART1, ART1, KIAA0764, SPT7L, STAF65, STAF65(gamma), STAF65gamma, STAGA complex 65 subunit gamma, Suppressor of Ty 7 like, SUPT7L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue, human gliomas tissue, rat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SUPT7L Ready-To-Use IHC Kit. SUPT7L primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25606-1-AP targets SUPT7L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 SUPT7L fusion protein Ag22264 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppressor of Ty 7 (S. cerevisiae)-like
别名 Adenocarcinoma antigen ART1, ART1, KIAA0764, SPT7L, STAF65, STAF65(gamma), STAF65gamma, STAGA complex 65 subunit gamma, Suppressor of Ty 7 like, SUPT7L
计算分子量 414 aa, 46 kDa
观测分子量 65 kDa
GenBank蛋白编号BC074000
基因名称 SUPT7L
Gene ID (NCBI) 9913
RRIDAB_2880156
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94864
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUPT7L, also named as KIAA0764, STAF65gamma, is a component of the STAGA transcription coactivator-HAT complex. The MW of SUPT7L is 46-65 kDa.

实验方案

Product Specific Protocols
WB protocol for SUPT7L antibody 25606-1-APDownload protocol
IHC protocol for SUPT7L antibody 25606-1-APDownload protocol
IF protocol for SUPT7L antibody 25606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Genet

Loss-of-function variants affecting the STAGA complex component SUPT7L cause a developmental disorder with generalized lipodystrophy

Authors - Johannes Kopp
View Article