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SURF1 Recombinant antibody, PBS Only (Detector)

SURF1 Recombinant Antibody for IP, IF, FC, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, IF, FC, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230210F11

Cat No : 83052-5-PBS

Print datasheet

Synonyms

SURF 1, SURF1, surfeit 1, Surfeit locus protein 1

验证数据展示

  • IP experiment of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

    IP experiment of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

    IP result of anti-SURF1 (IP:83052-5-RR, 4ug; Detection:83052-5-RR 1:2000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)

    IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)

    IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)

    IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)

    IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

    IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

    IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83052-5-RR (same clone as 83052-5-PBS)

    FC experiment of HepG2 using 83052-5-RR (same clone as 83052-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SURF1 Recombinant antibody (83052-5-RR, Clone:230210F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00034-3

    Cytometric bead array standard curve of MP00034-3

    Cytometric bead array standard curve ofMP00034-3, SURF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83052-2-PBS. Detection antibody: 83052-5-PBS. Standard: Ag3959. Range: 0.625-20 ng/mL.

产品信息

83052-5-PBS targets SURF1 as part of a matched antibody pair:

MP00034-3: 83052-2-PBS capture and 83052-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IP, IF, FC, IHC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen SURF1 fusion protein Ag3959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name surfeit 1
Synonyms SURF 1, SURF1, surfeit 1, Surfeit locus protein 1
Calculated Molecular Weight 33 kDa
GenBank Accession NumberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15526
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.