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SURF2 Polyclonal antibody

SURF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16522-1-AP

Print datasheet

Synonyms

surfeit 2, Surf-2, SURF 2

验证数据展示

  • WB analysis using 16522-1-AP

    WB analysis using 16522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 16522-1-AP

    IHC staining of human breast cancer using 16522-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16522-1-AP (SURF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16522-1-AP

    IHC staining of human breast cancer using 16522-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16522-1-AP (SURF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16522-1-AP

    IF Staining of HeLa using 16522-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16522-1-AP (SURF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16522-1-AP targets SURF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SURF2 fusion protein Ag9745 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name surfeit 2
Synonyms surfeit 2, Surf-2, SURF 2
Calculated Molecular Weight 256 aa, 30 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014411
Gene Symbol SURF2
Gene ID (NCBI) 6835
RRIDAB_2255415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15527
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SURF2 antibody 16522-1-APDownload protocol
IHC protocol for SURF2 antibody 16522-1-APDownload protocol
IF protocol for SURF2 antibody 16522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols