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SURF4 Polyclonal antibody

SURF4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11599-1-AP

Print datasheet

Synonyms

ERV29, SURF 4, SURF4, surfeit 4, Surfeit locus protein 4

验证数据展示

  • IHC staining of human breast cancer using 11599-1-AP

    IHC staining of human breast cancer using 11599-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11599-1-AP (SURF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 11599-1-AP

    WB analysis using 11599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11599-1-AP (SURF4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

11599-1-AP targets SURF4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SURF4 fusion protein Ag2175 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name surfeit 4
Synonyms ERV29, SURF 4, SURF4, surfeit 4, Surfeit locus protein 4
Calculated Molecular Weight 269 aa, 30 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018741
Gene Symbol SURF4
Gene ID (NCBI) 6836
RRIDAB_2877782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15260
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SURF4 antibody 11599-1-APDownload protocol
IHC protocol for SURF4 antibody 11599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Transl Med

Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment

Authors - Guohong Liu
View Article