验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse small intestine tissue, C6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10574-1-AP targets SUV39H1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SUV39H1 fusion protein Ag0854 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | suppressor of variegation 3-9 homolog 1 (Drosophila) |
| 别名 | H3 K9 HMTase 1, KMT1A, Lysine N methyltransferase 1A, MG44, Su(var)3 9 homolog 1, SUV39H, SUV39H1 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC006238 |
| 基因名称 | SUV39H1 |
| Gene ID (NCBI) | 6839 |
| RRID | AB_2196710 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43463 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUV39H1 antibody 10574-1-AP | Download protocol |
| IP protocol for SUV39H1 antibody 10574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication | ||
Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.
| ||
J Med Chem Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis. | ||
Cell Death Dis Dicer promotes tumorigenesis by translocating to nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells. | ||
Oncotarget Downregulation of HP1α suppresses proliferation of cholangiocarcinoma by restoring SFRP1 expression. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. |
