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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83646-6-RR (same clone as 83646-6-PBS)

    WB analysis using 83646-6-RR (same clone as 83646-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83646-6-RR (SWAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

    IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

    IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • FC experiment of SK-N-SH using 83646-6-RR (same clone as 83646-6-PBS)

    FC experiment of SK-N-SH using 83646-6-RR (same clone as 83646-6-PBS)

    1x10^6 SK-N-SH cells were intracellularly stained with 0.25 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83646-6-RR

    Affinity and Kinetic Characterization of 83646-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83646-6-RR against Human SWAP70 were performed. The affinity constant is below 1 pM.

SWAP70 Recombinant antibody, PBS Only

SWAP70 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83646-6-PBS

产品说明书

CloneNo. 240641F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

SWAP-70, SWAP 70 protein, SWAP 70, KIAA0640, HSPC321

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83646-6-PBS targets SWAP70 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 SWAP70 fusion protein Ag34855 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWAP-70 protein
别名 SWAP-70, SWAP 70 protein, SWAP 70, KIAA0640, HSPC321
计算分子量 69 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC000616
基因名称 SWAP70
Gene ID (NCBI) 23075
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UH65
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).