验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 4 publications below |
产品信息
11063-1-AP targets SYCE1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | SYCE1 fusion protein Ag1527 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | synaptonemal complex central element protein 1 |
| Synonyms | CT76, Cancer/testis antigen 76, C10orf94 |
| Calculated Molecular Weight | 315 aa, 36 kDa |
| Observed Molecular Weight | 40-44 kDa |
| GenBank Accession Number | BC034821 |
| Gene Symbol | SYCE1 |
| Gene ID (NCBI) | 93426 |
| RRID | AB_2197057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N0S2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Synaptonemal Complex Central Element 1 (Syce1) is a major component of the synaptonemal complex (SC), which is formed between homologous chromosomes during meiotic prophase and exists only during the first meiotic division. The SYCE1 structural core, formed by an N-terminal region of amino acids 25-179, is an α-helical dimer that adopts an anti-parallel "coiled-coil"-like structure of approximately 20 nm in length (PMID: 30607510). The male Syce1 null mice had abundant primary spermatocytes and lacked postmeiotic cells; arrested cells were likely eliminated by accelerated apoptosis ( PMID: 25899990).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SYCE1 antibody 11063-1-AP | Download protocol |
| IHC protocol for SYCE1 antibody 11063-1-AP | Download protocol |
| IF protocol for SYCE1 antibody 11063-1-AP | Download protocol |
| IP protocol for SYCE1 antibody 11063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility. | ||
Am J Hum Genet Homozygous mutations in C14orf39/SIX6OS1 cause non-obstructive azoospermia and premature ovarian insufficiency in humans. | ||
J Steroid Biochem Mol Biol Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells. | ||