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  • Featured Product
  • KD/KO Validated

SYF2 Polyclonal antibody

SYF2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16958-1-AP

Print datasheet

Synonyms

Pre-mRNA-splicing factor SYF2, Pre mRNA splicing factor SYF2, GCIPIP, CCNDBP1-interactor, CCNDBP1 interactor

验证数据展示

  • WB analysis of HEK-293 using 16958-1-AP

    WB analysis of HEK-293 using 16958-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16958-1-AP (SYF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 16958-1-AP

    IF Staining of HEK-293 using 16958-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 16958-1-AP (SYF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16958-1-AP targets SYF2 in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, RIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen SYF2 fusion protein Ag10656 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SYF2 homolog, RNA splicing factor (S. cerevisiae)
Synonyms Pre-mRNA-splicing factor SYF2, Pre mRNA splicing factor SYF2, GCIPIP, CCNDBP1-interactor, CCNDBP1 interactor
Calculated Molecular Weight 243 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC010862
Gene Symbol SYF2
Gene ID (NCBI) 25949
RRIDAB_2197205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SYF2 antibody 16958-1-APDownload protocol
IF protocol for SYF2 antibody 16958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,RIP

Nucleic Acids Res

ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.

Authors - Iris Tanaka
  • KD Validated
View Article