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  • Featured Product
  • KD/KO Validated

SYK Polyclonal antibody

SYK Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22206-1-AP

Print datasheet

Synonyms

Tyrosine-protein kinase SYK, Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82

验证数据展示

  • WB analysis of HEK-293 using 22206-1-AP

    WB analysis of HEK-293 using 22206-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22206-1-AP (SYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 22206-1-AP

    IP experiment of HEK-293 using 22206-1-AP

    IP result of anti-SYK (IP:22206-1-AP, 4ug; Detection:22206-1-AP 1:500) with HEK-293 cells lysate 2700ug.

  • IHC staining of human spleen using 22206-1-AP

    IHC staining of human spleen using 22206-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 22206-1-AP

    IHC staining of human spleen using 22206-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 40x lens).

  • FC experiment of Ramos using 22206-1-AP

    FC experiment of Ramos using 22206-1-AP

    1x10^6 Ramos cells were intracellularly stained with 0.4 ug SYK Polyclonal antibody (22206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inRamos cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22206-1-AP targets SYK in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SYK fusion protein Ag17526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spleen tyrosine kinase
Synonyms Tyrosine-protein kinase SYK, Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82
Calculated Molecular Weight 635 aa, 72 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
RRIDAB_2879028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

实验方案

Product Specific Protocols
WB protocol for SYK antibody 22206-1-APDownload protocol
IHC protocol for SYK antibody 22206-1-APDownload protocol
IP protocol for SYK antibody 22206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Virulence

Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK.

Authors - Xuelin Han
View Article
mouseWB

Biochim Biophys Acta

Syk negatively regulates TLR4-mediated IFNβ and IL-10 production and promotes inflammatory responses in dendritic cells.

Authors - Hui Yin
  • KO Validated
View Article