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验证数据展示

  • WB analysis using 30549-1-AP

    WB analysis using 30549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30549-1-AP (SYN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 30549-1-AP

    IHC staining of mouse pancreas using 30549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 30549-1-AP (SYN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYN1 Polyclonal antibody

SYN1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30549-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Synapsin I, Synapsin1, Synapsin 1, SYN1b, SYN1a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inIMR-32 cells, SH-SY5Y cells, SK-N-SH cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30549-1-AP targets SYN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SYN1 fusion protein Ag31091 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synapsin I
Synonyms Synapsin I, Synapsin1, Synapsin 1, SYN1b, SYN1a
Calculated Molecular Weight 74 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberNM_006950
Gene Symbol SYN1
Gene ID (NCBI) 6853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17600
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYN1, also named Synapsin I, Brain protein 4.1, belongs to the Synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to regulate neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD). Synapsin I (SYN1) is composed of two isoforms, synapsin Ia and Ib, with molecular weights of 74 kDa and 70 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for SYN1 antibody 30549-1-APDownload protocol
IHC protocol for SYN1 antibody 30549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols