验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, THP-1 cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30443-1-AP targets SYNCRIP in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | SYNCRIP fusion protein Ag31509 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | synaptotagmin binding, cytoplasmic RNA interacting protein |
别名 | dJ3J17.2, GRY RBP, hnRNP Q, HNRPQ, HNRPQ1, NS1 associated protein 1, NSAP1, pp68, RP1 3J17.2, SYNCRIP |
计算分子量 | 623 aa, 70 kDa |
观测分子量 | 62-74 kDa |
GenBank蛋白编号 | BC032643 |
基因名称 | SYNCRIP |
Gene ID (NCBI) | 10492 |
RRID | AB_3086317 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O60506 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Synaptotagmin-binding, cytoplasmic RNA-interacting protein(SYNCRIP), also identified as hnRNP Q, involves in mRNA processing mechanisms. It's component of the CRD-mediated complex that promotes MYC mRNA stability. Also it is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. As previously reported, the 65 and 74 kDa proteins corresponded to two isoforms of SYNCRIP produced from alternatively spliced mRNAs (PMID: 15340051).
实验方案
Product Specific Protocols | |
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WB protocol for SYNCRIP antibody 30443-1-AP | Download protocol |
IF protocol for SYNCRIP antibody 30443-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |