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SYNCRIP Polyclonal antibody

SYNCRIP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30443-1-AP

Print datasheet

Synonyms

dJ3J17.2, GRY RBP, hnRNP Q, HNRPQ, HNRPQ1, NS1 associated protein 1, NSAP1, pp68, RP1 3J17.2, SYNCRIP

验证数据展示

  • WB analysis using 30443-1-AP

    WB analysis using 30443-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30443-1-AP (SYNCRIP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30443-1-AP

    IF Staining of A431 using 30443-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SYNCRIP antibody (30443-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inK-562 cells, THP-1 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SYNCRIP Ready-To-Use IHC Kit. SYNCRIP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30443-1-AP targets SYNCRIP in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen SYNCRIP fusion protein Ag31509 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin binding, cytoplasmic RNA interacting protein
Synonyms dJ3J17.2, GRY RBP, hnRNP Q, HNRPQ, HNRPQ1, NS1 associated protein 1, NSAP1, pp68, RP1 3J17.2, SYNCRIP
Calculated Molecular Weight 623 aa, 70 kDa
Observed Molecular Weight62-74 kDa
GenBank Accession NumberBC032643
Gene Symbol SYNCRIP
Gene ID (NCBI) 10492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60506
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptotagmin-binding, cytoplasmic RNA-interacting protein(SYNCRIP), also identified as hnRNP Q, involves in mRNA processing mechanisms. It's component of the CRD-mediated complex that promotes MYC mRNA stability. Also it is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. As previously reported, the 65 and 74 kDa proteins corresponded to two isoforms of SYNCRIP produced from alternatively spliced mRNAs (PMID: 15340051).

实验方案

Product Specific Protocols
WB protocol for SYNCRIP antibody 30443-1-APDownload protocol
IF protocol for SYNCRIP antibody 30443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols