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Nesprin 2 Polyclonal antibody

Nesprin 2 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 25265-1-AP

Print datasheet

Synonyms

KIAA1011, Nesprin 2, NUA, NUANCE, Protein NUANCE, SYNE 2, SYNE2

验证数据展示

  • IF Staining of Human dorsal cerebral organoid using 25265-1-AP

    IF Staining of Human dorsal cerebral organoid using 25265-1-AP

    Human dorsal cerebral organoid at 12 weeks. 4% PFA fixed. Antibody dilution was 1:500 for Syne-2 (25265-1-AP; white), DAPI 1:1000. Image created by Wimmer Ryszard from the Institut Curie in Paris, France

  • WB analysis of HEK-293 using 25265-1-AP

    WB analysis of HEK-293 using 25265-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25265-1-AP (SYNE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 25265-1-AP

    IF Staining of mouse brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 25265-1-AP

    IF Staining of mouse brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of rat brain using 25265-1-AP

    IF Staining of rat brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF detected inHuman dorsal cerebral organoid, mouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25265-1-AP targets Nesprin 2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spectrin repeat containing, nuclear envelope 2
Synonyms KIAA1011, Nesprin 2, NUA, NUANCE, Protein NUANCE, SYNE 2, SYNE2
Calculated Molecular Weight 799 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberNM_015180
Gene Symbol SYNE2
Gene ID (NCBI) 23224
RRIDAB_2879995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.

实验方案

Product Specific Protocols
WB protocol for Nesprin 2 antibody 25265-1-APDownload protocol
IF protocol for Nesprin 2 antibody 25265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols