• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of Human dorsal cerebral organoid using 25265-1-AP

    IF Staining of Human dorsal cerebral organoid using 25265-1-AP

    Human dorsal cerebral organoid at 12 weeks. 4% PFA fixed. Antibody dilution was 1:500 for Syne-2 (25265-1-AP; white), DAPI 1:1000. Image created by Wimmer Ryszard from the Institut Curie in Paris, France

  • WB analysis of HEK-293 using 25265-1-AP

    WB analysis of HEK-293 using 25265-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25265-1-AP (SYNE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 25265-1-AP

    IF Staining of mouse brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 25265-1-AP

    IF Staining of mouse brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of rat brain using 25265-1-AP

    IF Staining of rat brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Nesprin 2 Polyclonal antibody

Nesprin 2 Polyclonal Antibody for WB, IF, ELISA
Cat No. 25265-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, ELISA

别名

SYNE2, SYNE 2, NUANCE, NUA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF detected inHuman dorsal cerebral organoid, mouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25265-1-AP targets Nesprin 2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spectrin repeat containing, nuclear envelope 2
别名 SYNE2, SYNE 2, NUANCE, NUA
计算分子量 799 kDa
观测分子量 50-60 kDa
GenBank蛋白编号NM_015180
基因名称 Nesprin 2
Gene ID (NCBI) 23224
RRIDAB_2879995
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXH0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.

实验方案

Product Specific Protocols
WB protocol for Nesprin 2 antibody 25265-1-APDownload protocol
IF protocol for Nesprin 2 antibody 25265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer

Authors - Bei-Bei Sun
View Article