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验证数据展示

  • WB analysis using 10432-1-AP

    WB analysis using 10432-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10432-1-AP (SYNGR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 10432-1-AP

    IF Staining of A431 using 10432-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SYNGR1 antibody (10432-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SYNGR1 Polyclonal antibody

SYNGR1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10432-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, pig

应用

WB, IF/ICC, ELISA

别名

Synaptogyrin-1, synaptogyrin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, pig brain
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10432-1-AP targets SYNGR1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat, pig
免疫原 SYNGR1 fusion protein Ag0685 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptogyrin 1
别名 Synaptogyrin-1, synaptogyrin 1
计算分子量 25 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC000731
基因名称 SYNGR1
Gene ID (NCBI) 9145
RRIDAB_2200874
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43759
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)

实验方案

Product Specific Protocols
WB protocol for SYNGR1 antibody 10432-1-APDownload protocol
IF protocol for SYNGR1 antibody 10432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols