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SYNGR1 Monoclonal antibody

SYNGR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H2H1

Cat no : 68176-1-Ig

Print datasheet

Synonyms

Synaptogyrin-1, synaptogyrin 1, 2H2H1

验证数据展示

  • WB analysis using 68176-1-Ig

    WB analysis using 68176-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of rat brain using 68176-1-Ig

    IHC staining of rat brain using 68176-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 68176-1-Ig

    IHC staining of rat brain using 68176-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 68176-1-Ig

    IF Staining of mouse brain using 68176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNGR1 antibody (68176-1-Ig, Clone: 2H2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of mouse retina using 68176-1-Ig

    WB analysis of mouse retina using 68176-1-Ig

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse retina tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68176-1-Ig targets SYNGR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen SYNGR1 fusion protein Ag31309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name synaptogyrin 1
Synonyms Synaptogyrin-1, synaptogyrin 1, 2H2H1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC000731
Gene Symbol SYNGR1
Gene ID (NCBI) 9145
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43759
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)

实验方案

Product Specific Protocols
WB protocol for SYNGR1 antibody 68176-1-IgDownload protocol
IHC protocol for SYNGR1 antibody 68176-1-IgDownload protocol
IF protocol for SYNGR1 antibody 68176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols